| 论文题目: | A functional recT gene for recombineering of Clostridium |
|---|---|
| 作者: | Dong Hongjun, Tao Wenwen, Gong Fuyu, Li Yin, Zhang Yanping* |
| 联系作者: | Zhang Yanping* |
| 刊物名称: | Journal of Biotechnology |
| 期: | 173 |
| 卷: | |
| 页: | 65-67 |
| 年份: | 2014 |
| 影响因子: | 3.34 |
| 论文下载: | |
| 摘要: | Recombineering is an efficient genetic manipulation method employing the mechanism of phagenic RecT-mediated homologous recombination. To develop a recombineering method for Clostridium, a putative recT gene (CPF0939) from Clostridium perfringens genome was functionally verified in a clostridial host Clostridium acetobutylicum. We show that a short synthetic oligonucleotide can be introduced into the target site for specific point mutation. This functional recT gene would therefore contribute to development of recombineering tools for Clostridium. (C) 2014 Elsevier B.V. All rights reserved. |
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