Successful bioremediation of an aged and heavily contaminated soil using a microbial/plant combination strategy----中国科学院微生物研究所

科研成果

论文题目:	Successful bioremediation of an aged and heavily contaminated soil using a microbial/plant combination strategy
作者:	Xu Yang, Sun GuangDong, Jin JingHua, Liu Ying, Luo Mu, Zhong ZhiPing, Liu ZhiPei*
联系作者:	Liu ZhiPei*
刊物名称:	Journal of Hazardous Materials
期:
卷:	264C
页:	430-438
年份:	2013
影响因子:	4.679
论文下载:	http://www.sciencedirect.com/science/article/pii/S0304389413008224
摘要:	Bioremediation of an aged and heavily contaminated soil was performed using microbial remediation, phytoremediation, and microbial/phytoremediation. The removal efficiency of polycyclic aromatic hydrocarbons (PAHs) was in the order microbial/phytoremediation>microbial remediation approximately phytoremediation>control. The removal percentage of microbial/phytoremediation (69.6%) was twice that of control. Kocuria sp. P10 significantly enhanced PAH removal (P<0.05) and ryegrass growth (P<0.01). Dehydrogenase activity increased steadily and was negatively correlated with total PAH content. Successional changes in soil microbial communities were also detected by pyrosequencing. The results indicated that biodiversity of the soil bacterial community gradually increased with time and was slightly lower in control, as indicated by operational taxonomic unit (OTU) numbers and Shannon-Wiener indices. Proportions of Betaproteobacteria and Gammaproteobacteria were consistently high in all groups. Actinobacteridae were initially predominant (>37.8%) but rapidly decreased to <4%. The proportions of Acidobacteria increased greatly and this increase was positively correlated with PAH removal. These findings indicate a healthy ecological progression and a role of Acidobacteria as an indicator of the process. This study provides new insights into the dynamics of community structure during bioremediation process and a possible basis for ecological assessment for bioremediation on a large scale.