Identification of the First Diphenyl Ether Gene Cluster for Pestheic Acid Biosynthesis in Plant Endophyte Pestalotiopsis fici----中国科学院微生物研究所

科研成果

论文题目:	Identification of the First Diphenyl Ether Gene Cluster for Pestheic Acid Biosynthesis in Plant Endophyte Pestalotiopsis fici
作者:	Xu Xinxin#, Liu Ling#, Zhang Fan, Wang Wenzhao, Li Jinyang, Guo Liangdong, Che Yongsheng, Liu Gang
联系作者:	Che Yongsheng, Liu Gang
刊物名称:	Chembiochem
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年份:	2013
影响因子:	3.67
论文下载:	http://onlinelibrary.wiley.com/doi/10.1002/cbic.201300626/abstract;jsessionid=2F5910F34FCCA5C2DF0FAFB818FD99E4.f03t04
摘要:	The diphenyl ether pestheic acid was isolated from the endophytic fungus Pestalotiopsis fici, which is proposed to be the biosynthetic precursor of the unique chloropupukeananes. The pestheic acid biosynthetic gene (pta) cluster was identified in the fungus through genome scanning. Sequence analysis revealed that this gene cluster encodes a nonreducing polyketide synthase, a number of modification enzymes, and three regulators. Gene disruption and intermediate analysis demonstrated that the biosynthesis proceeded through formation of the polyketide backbone, cyclization of a polyketo acid to a benzophenone, chlorination, and formation of the diphenyl ether skeleton through oxidation and hydrolyzation. A dihydrogeodin oxidase gene, ptaE, was essential for diphenyl ether formation, and ptaM encoded a flavin-dependent halogenase catalyzing chlorination in the biosynthesis. Identification of the pta cluster laid the foundation to decipher the genetic and biochemical mechanisms involved in the pathway.