Methanospirillum psychrodurum sp. nov., isolated from the wetland soil of Qinghai-Tibetan Plateau, China----中国科学院微生物研究所

科研成果

论文题目:	Methanospirillum psychrodurum sp. nov., isolated from the wetland soil of Qinghai-Tibetan Plateau, China
作者:	Zhou Liguang, Liu Xiaoli, Dong Xiuzhu*
联系作者:	Dong Xiuzhu*
刊物名称:	International Journal of Systematic and Evolutionary Microbiology
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卷:
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年份:	2013
影响因子:	2.347
论文下载:	http://ijs.sgmjournals.org/content/early/2013/10/22/ijs.0.057299-0.long
摘要:	A psychrotolerant methanogenic strain X-18T, was isolated from the soil of Madoi wetland at Qinghai-Tibetan plateau, China. Cells were wavy rods (11-62 mum long) with blunt tapered ends and Gram-negative stained. Strain X-18T grew strict anaerobically and produced methane exclusively from H2/CO2. Growth occurred in the temperature range of 4-32 degrees C and optimally at 25 degrees C. Growth pH ranged from 6.5-8.0 and optimally at 7.0. The G+C content of the genomic DNA of strain X-18T was 44.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences and the alpha subunit of methyl-coenzyme M reductase indicated that strain X-18T affiliated to the genus Methanospirillium, and was most related to Methanospirillum lacunae Ki8-1T at 96.3% 16S rRNA gene sequence similarity. However, strain X-18T was distinguished from the existing Methanospirillium spp. by the lower growth temperature and obligate hydrogenotrophic methanogenesis. On the basis of phenotypic characteristics and phylogenetic analysis, strain X-18T can represent a novel species of the genus Methanospirillum, for which Methanospirillum psychrodurum sp. nov. is proposed and strain X-18T is assigned as the type strain (=CGMCC 1.5186T=JCM 19216T).