| 论文题目: | Development of conventional and nested PCR assays for the detection of Ophiocordyceps sinensis |
|---|---|
| 作者: | Jin Guosheng#, Wang Xiaoliang#, Li Yi, Wang Wenjing, Yang Ruiheng, Ren Shuyu, Yao Yijian* |
| 联系作者: | Yao Yijian* |
| 刊物名称: | Journal of Basic Microbiology |
| 期: | 4 |
| 卷: | 53 |
| 页: | 340-7 |
| 年份: | 2013 |
| 影响因子: | 1.536 |
| 论文下载: | http://onlinelibrary.wiley.com/doi/10.1002/jobm.201200029/pdf |
| 摘要: | Ophiocordyceps sinensis, endemic to the Tibetan Plateau, is one of the most important medicinal fungi with a huge economic value. In the present study, specific primer pairs were designed based on a comprehensive ITS sequence dataset of O. sinensis and its related fungi, and tested for specificity and sensitivity through PCR experiments using 27 individuals of O. sinensis from different geographical origins and 40 other related fungal species in terms of phylogeny or ecology. A primer pair highly specific to O. sinensis was obtained, yielding a 275 bp PCR product from all the individuals of O. sinensis but no product from the other fungi tested. The detection limit of the primers was demonstrated to be 10 ng of pure O. sinensis DNA for conventional PCR and 10 pg for nested PCR in a 25 microl reaction system. Soil samples collected from the habitat of O. sinensis were also tested using this PCR assay. The results showed that the primer pair and PCR-based assays developed in this study can be applied to the rapid detection of O. sinensis in its natural habitat. |
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