Efficient production of polymer-grade L-lactate by an alkaliphilic Exiguobacterium sp strain under nonsterile open fermentation conditions----中国科学院微生物研究所

科研成果

论文题目:	Efficient production of polymer-grade L-lactate by an alkaliphilic Exiguobacterium sp strain under nonsterile open fermentation conditions
作者:	Jiang Xu, Xue Yanfen, Wang Aiyan, Wang Limin, Zhang Guimin, Zeng Qingtao, Yu Bo*, Ma Yanhe
联系作者:	Yu Bo*
刊物名称:	Bioresource Technology
期:
卷:	143
页:	665-668
年份:	2013
影响因子:	5.172
论文下载:	http://www.sciencedirect.com/science/article/pii/S0960852413009681
摘要:	In this study, a newly isolated alkaliphile Exiguobacterium sp. strain 8-11-1 was used to produce optically pure L-lactate. With an initial glucose concentration of 80 g/L, a high overall L-lactic acid productivity of 8.15 g/L/h was achieved using NaOH as a neutralizing agent. The fed-batch fermentations were carried out under both sterile and nonsterile conditions. Under the nonsterile condition, 125 g/L L-lactic acid was obtained with a high percent yield and average productivity of 98.33% and 3.79 g/L/h, respectively. These values were consistent with the results from sterile condition. No D-isomers of lactic acid were detected, resulting in an optical purity of 100% in both conditions. The high levels of optically pure L-lactic acid produced by Exiguobacterium sp. 8-11-1, combined with the ease of handling and low costs associated with the open fermentation strategy, provide a novel and potentially important approach for L-lactic acid production in the future. (C) 2013 Elsevier Ltd. All rights reserved.