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论文题目: Mycetocola miduiensis sp nov., a psychrotolerant bacterium isolated from Midui glacier
作者: Zhu Lang, Liu Qing, Liu Hongcan, Zhou Yuguang, Xin Yuhua*, Dong Xiuzhu*
联系作者: Xin Yuhua*, Dong Xiuzhu*
刊物名称: International Journal of Systematic and Evolutionary Microbiology
期:
卷: 63
页: 2661-2665
年份: 2013
影响因子: 2.347
论文下载: http://ijs.sgmjournals.org/content/63/Pt_7/2661.long
摘要: An aerobic, asporous, flagellated, Gram-stain-positive, rod-shaped bacterium MD-T1-10-2(T) was isolated from the topsoil of Midui Glacier, Tibet Province, China. Phylogenetic analysis based on 16S rRNA gene sequence analysis placed the strain in a clade containing Mycetocola manganoxydans CCTCC AB 209002(T), Mycetocola reblochoni DSM 18580(T), Mycetocola tolaasinivorans JCM 11656(T), Mycetocola lacteus JCM 11654(T) and Mycetocola saprophilus JCM 11655(T), with the sequence similarities of 99.2, 98.1, 96.7, 96.6 and 96.4%, respectively. DNA- DNA hybridization analysis indicated that strain MD-T1-10-2(T) represented a new member of this genus. The optimal ranges of temperature and pH for growth were 20-25 degrees C and 7.0-9.0, respectively; the strain could even grow at 0 degrees C. The major cellular fatty acids were anteiso-C-15:0, iso-C-16:0 and anteiso-C-17:0. The predominant menaquinones were MK-10 and MK-11. The cell wall amino acids were lysine, alanine, glycine and glutamic acids. The DNA G+C content was 65.9 mol%. Based on the genotypic and phenotypic data, strain MD-T1-10-2T for which the name Mycetocola miduiensis sp. nov. is proposed; the type strain is MD-T1-10-2(T) (=CGMCC 1.11101(T)=NBRC 107877(T)).