Inhibition effect of enteropeptidase on RANKL-RANK signalling by cleavage of RANK----中国科学院微生物研究所

科研成果

论文题目:	Inhibition effect of enteropeptidase on RANKL-RANK signalling by cleavage of RANK
作者:	Zhao Yunfeng, Jin Mengmeng, Ma Juan, Zhang Shiqian, Li Wei, Chen Yuan, Zhou Yingsheng, Tao Hong, Liu Yu, Wang Lei, Han Huamin, Niu Ge, Tao Hua, Liu Changzhen, Gao Bin
联系作者:	Liu Changzhen, Gao Bin
刊物名称:	FEBS Lett
期:	18
卷:	587
页:	2958-2964
年份:	2013
影响因子:	3.478
论文下载:	http://www.sciencedirect.com/science/article/pii/S001457931300611X
摘要:	Enteropeptidase can cleave trypsinogen on the sequence of Asp-Asp-Asp-Asp-Lys and plays an important role in food digestion. The RANKL-RANK signalling pathway plays a pivotal role in bone remodelling. In this study, we reported that enteropeptidase can inhibit the RANKL-RANK signalling pathway through the cleavage of RANK. A surrogate peptide blocking assay indicated that enteropeptidase could specifically cleave RANK on the sequence NEEDK. Osteoclast differentiation assay and NF-kappa B activity assay confirmed that enteropeptidase could inhibit osteoclastogenesis in vitro through the cleavage of RANK. This is the first study to prove that the RANKL-RANK signalling pathway can be inhibited by cleavage of RANK instead of targeting RANKL. Structured summary of protein interactions: EP cleaves hRANK by cleavage assay (View interaction) EP cleaves mRANK by cleavage assay (View interaction) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.