Bacillus sp. strain P38: An efficient producer of l-lactate from cellulosic hydrolysate, with high tolerance for 2-furfural----中国科学院微生物研究所

科研成果

论文题目:	Bacillus sp. strain P38: An efficient producer of l-lactate from cellulosic hydrolysate, with high tolerance for 2-furfural
作者:	Peng Lili, Wang Limin, Che Chengchuan, Yang Ge, Yu Bo*, Ma Yanhe
联系作者:	Yu Bo*
刊物名称:	Bioresource Technology
期:
卷:
页:	169-176
年份:	2013
影响因子:	5.172
论文下载:	http://www.sciencedirect.com/science/article/pii/S0960852413014788
摘要:	In this study, efficient polymer-grade l-lactic acid production was achieved with the strain Bacillus sp. P38 by using cellulosic hydrolysate as the sole carbon source. In fed-batch fermentation, 180gL-1l-lactic acid was obtained with a volumetric productivity of 2.4gL-1h-1 and a yield of 0.96gg-1 total reducing sugars. No d-isomer of lactic acid was detected in the broth. Strain P38 tolerated up to 10gL-1 2-furfural, and lactate production was sharply inhibited only when the 2-furfural concentration was higher than 6gL-1. Moreover, strain P38 also tolerated high concentrations (>6gL-1) of other fermentation inhibitors in cellulosic hydrolysate, such as vanillin and acetic acid, although it was slightly sensitive to formic acid. The efficient l-lactic acid production, combined with high inhibitor tolerance and efficient pentose utilization, indicate that Bacillus sp. P38 is a promising producer of polymer-grade l-lactic acid from cellulosic biomass.