论文题目: | Efficient production of polymer-grade d-lactate by Sporolactobacillus laevolacticus DSM442 with agricultural waste cottonseed as the sole nitrogen source |
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作者: | Li Yuan, Wang Limin, Ju Jiansong, Yu Bo*, Ma Yanhe |
联系作者: | Yu Bo |
刊物名称: | Bioresource Technology |
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年份: | 2013 |
影响因子: | 5.352 |
论文下载: | http://www.sciencedirect.com/science/article/pii/S0960852413007578 |
摘要: | In this study, we show that Sporolactobacillus laevolacticus DSM442 can produce d-lactate by using cottonseed as the sole nitrogen source. The cottonseed was enzymatically hydrolyzed and simultaneously utilized during d-lactate fermentation. Under optimal conditions, a high d-lactic acid concentration (144.4g/L) was obtained in a fed-batch fermentation within 35h, with an average productivity of 4.13g/(Lh) and a yield of 0.96g/g glucose. The optical purity of d-lactic acid in the broth was 99.3%, which meets the requirement for use in lactic acid polymerization. Our study represents a cost-effective method for polymer-grade d-lactate production using cheap agricultural wastes. |
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