| 论文题目: | Glycosynthase with Broad Substrate Specificity – an Efficient Biocatalyst for the Construction of Oligosaccharide Library. |
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| 作者: | Wei Jinhua, Lv Xun, Lü Yang, Yang Gangzhu, Fu Lifeng, Yang Liu, Wang Jianjun, Gao Jianhui, Cheng Shuihong, Duan Qian, Jin Cheng, Li Xuebing |
| 联系作者: | Li Xuebing |
| 刊物名称: | European Journal of Organic Chemistry |
| 期: | 12 |
| 卷: | 2013 |
| 页: | 2414-2419 |
| 年份: | 2013 |
| 影响因子: | 3.289 |
| 论文下载: | |
| 摘要: | A versatile glycosynthase (TnG-E338A) with strikingly broad substrate scope has been developed from Thermus nonproteolyticus β-glycosidase (TnG) by using site-directed mutagenesis. The practical utility of this biocatalyst has been demonstrated by the facile generation of a small library containing various oligosaccharides and a steroidal glycoside (total 25 compounds) in up to 100 % isolated yield. Moreover, an array of eight gluco-oligosaccharides has been readily synthesized by the enzyme in a one-pot, parallel reaction, which highlights its potential in the combinatorial construction of a carbohydrate library that will assist glycomic and glycotherapeutic research. Significantly, the enzyme provides a means by which glycosynthase technology may be extended to combinatorial chemistry. |
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