| 论文题目: | Pentamerisation of a scFv directed against TRAIL receptor 2 increases its antitumour efficacy |
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| 作者: | Wang Wei, He Wen, Wang Lei, Zhang Ge, Gao Bin |
| 联系作者: | Gao Bin |
| 刊物名称: | Immunology and cell biology |
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| 年份: | 2013 |
| 影响因子: | 3.609 |
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| 摘要: | Single-chain variable fragments (scFvs) have been considered as promising therapeutic drugs. However, their low affinity and rapid clearance from the blood have limited their wider application clinically. In this study, we aimed to improve scFv antibodies by multimerising a scFv against the death receptor 5 (DR5, TNFR10B) through fusion with a coiled-coil domain of human cartilage oligomeric matrix protein 48. By forming a pentamer, nick-named a combody, the avidity of the scFv increased 104-fold and combody was more effective than its monomeric counterpart for antitumour activity in both in vitro and in vivo experiments.Immunology and Cell Biology advance online publication, 9 April 2013; doi:10.1038/icb.2013.10. |
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