| 论文题目: | Iterative Combinatorial Mutagenesis as an Effective Strategy for Generation of Deacetoxycephalosporin C Synthase with Improved Activity toward Penicillin G |
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| 作者: | Ji, JJ; Fan, KQ; Tian, XY; Zhang, X; Zhang, YX; Yang, KQ |
| 联系作者: | Yang, KQ |
| 刊物名称: | APPLIED AND ENVIRONMENTAL MICROBIOLOGY |
| 期: | 21 |
| 卷: | 78 |
| 页: | 7809-7812 |
| 年份: | 2012 |
| 影响因子: | 3.829 |
| 论文下载: | |
| 摘要: | An iterative combinatorial mutagenesis (ICM) strategy was used to engineer deacetoxycephalosporin C synthase of Streptomyces clavuligerus (scDAOCS) for improved activity toward penicillin G. Seven mutational sites were repeatedly combined onto a starter mutant (C155Y Y184H V275I C281Y) of scDAOCS. Eleven improved combinatorial mutants were identified from 24 mutants in four rounds of ICM. |
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