| 论文题目: | Construction of self-cloning, indigenous wine strains of Saccharomyces cerevisiae with enhanced glycerol and glutathione production |
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| 作者: | Hao, RY; Liu, YL; Wang, ZY; Zhang, BR |
| 联系作者: | Liu, YL |
| 刊物名称: | BIOTECHNOLOGY LETTERS |
| 期: | 9 |
| 卷: | 34 |
| 页: | 1711-1717 |
| 年份: | 2012 |
| 影响因子: | 1.683 |
| 论文下载: | |
| 摘要: | To improve wine taste and flavor stability, a novel indigenous strain of Saccharomyces cerevisiae with enhanced glycerol and glutathione (GSH) production for winemaking was constructed. ALD6 encoding an aldehyde dehydrogenases of the indigenous yeast was replaced by a GPD1 and CUP1 gene cassette, which are responsible for NAD-dependent glycerol-3-phosphatase dehydrogenase and copper resistance, respectively. Furthermore, the alpha-acetohydroxyacid synthase gene ILV2 of the indigenous yeast was disrupted by integration of the GSH1 gene which encodes gamma-glutamylcysteine synthetase and the CUP1 gene cassette. The fermentation capacity of the recombinant was similar to that of the wild-type strain, with an increase of 21 and 19 % in glycerol and GSH production. No heterologous DNA was harbored in the recombinant in this study. |
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