Purification, crystallization and preliminary X-ray analysis of the IgV domain of human nectin-4----中国科学院微生物研究所

科研成果

论文题目:	Purification, crystallization and preliminary X-ray analysis of the IgV domain of human nectin-4
作者:	Xu, X; Zhang, XA; Lu, GW; Cai, YP
联系作者:	Cai, YP
刊物名称:	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
期:
卷:	68
页:	942-945
年份:	2012
影响因子:	0.506
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摘要:	Nectin-4 belongs to a family of immunoglobulin-like cell adhesion molecules and is highly expressed in cancer cells. Recently, nectin-4 was found to be a receptor of measles virus and the IgV domain sustains strong binding to measles virus H protein. In this study, the successful expression and purification of human nectin-4 V domain (nectin-4v) is reported. The purified protein was crystallized using the sitting-drop vapour-diffusion method. The crystals diffracted to 1.8 angstrom resolution and belonged to space group P21, with unit-cell parameters a = 33.1, b = 51.7, c = 56.9 angstrom, beta = 94.7 degrees. Preliminary analysis of the diffraction data was also performed.