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论文题目: Expression of Bacterial GshF in Pichia pastoris for Glutathione Production
作者: Ge, SL; Zhu, TC; Li, Y
联系作者: Zhu, TC
刊物名称: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
期: 15
卷: 78
页: 5435-5439
年份: 2012
影响因子: 3.829
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摘要: Conventionally, two consecutive enzymatic reactions catalyzed by gamma-glutamylcysteine synthetase and glutathione synthetase are most commonly used for glutathione production. Here we demonstrate that bacterial bifunctional GshF can be used for glutathione production in a eukaryotic system without accumulation of the intermediate gamma-glutamylcysteine.