论文题目: | Expression of Bacterial GshF in Pichia pastoris for Glutathione Production |
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作者: | Ge, SL; Zhu, TC; Li, Y |
联系作者: | Zhu, TC |
刊物名称: | APPLIED AND ENVIRONMENTAL MICROBIOLOGY |
期: | 15 |
卷: | 78 |
页: | 5435-5439 |
年份: | 2012 |
影响因子: | 3.829 |
论文下载: | |
摘要: | Conventionally, two consecutive enzymatic reactions catalyzed by gamma-glutamylcysteine synthetase and glutathione synthetase are most commonly used for glutathione production. Here we demonstrate that bacterial bifunctional GshF can be used for glutathione production in a eukaryotic system without accumulation of the intermediate gamma-glutamylcysteine. |
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