| 论文题目: | Identification and Characterization of Two Functionally Unknown Genes Involved in Butanol Tolerance of Clostridium acetobutylicum |
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| 作者: | Jia, KZ; Zhang, YP; Li, Y |
| 联系作者: | Jia, KZ |
| 刊物名称: | PLOS ONE |
| 期: | 6 |
| 卷: | 7 |
| 页: | |
| 年份: | 2012 |
| 影响因子: | 4.092 |
| 论文下载: | |
| 摘要: | Solvents toxicity is a major limiting factor hampering the cost-effective biotechnological production of chemicals. In Clostridium acetobutylicum, a functionally unknown protein (encoded by SMB_G1518) with a hypothetical alcohol interacting domain was identified. Disruption of SMB_G1518 and/or its downstream gene SMB_G1519 resulted in increased butanol tolerance, while overexpression of SMB_G1518-1519 decreased butanol tolerance. In addition, SMB_G1518-1519 also influences the production of pyruvate: ferredoxin oxidoreductase (PFOR) and flagellar protein hag, the maintenance of cell motility. We conclude that the system of SMB_G1518-1519 protein plays a role in the butanol sensitivity/tolerance phenotype of C. acetobutylicum, and can be considered as potential targets for engineering alcohol tolerance. |
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