| 论文题目: | Isolation and identification of a novel polysaccharide-peptide complex with antioxidant, anti-proliferative and hypoglycaemic activities from the abalone mushroom |
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| 作者: | Li, N; Li, L; Fang, JC; Wong, JH; Ng, TB; Jiang, Y; Wang, CR; Zhang, NY; Wen, TY; Qu, LY; Lv, PY; Zhao, RL; Shi, B; Wang, YP; Wang, XY; Liu, F |
| 联系作者: | Ng, TB |
| 刊物名称: | BIOSCIENCE REPORTS |
| 期: | 3 |
| 卷: | 32 |
| 页: | 221-228 |
| 年份: | 2012 |
| 影响因子: | 2.379 |
| 论文下载: | |
| 摘要: | A novel antioxidant polysaccharide-peptide complex LB-1b from the fruiting bodies of the edible abalone mushroom (Pleurotus abalonus) was purified and identified. The structural characteristic of LB-1b was identified by FTIR (Fourier-transform IR), C-13 NMR and H-1 NMR spectroscopy. LB-1b is a polysaccharide peptide complex that contains glucose, rhamnose, glucuronic acid and galactose in the molar ratio of 22.4:1:1.7:1.6 and the N-terminal sequence of its peptide moiety has also been determined. The N-terminal amino acid sequence of LB-1b, IPKERKEFQQAQHLK, showed some resemblance to antioxidant enzymes. LB-1b exhibited high antioxidant activity in erythrocyte haemolysis in vitro and the anti-proliferative activity towards hepatoma HepG2 cells and breast cancer MCF7 cells with an IC50 of 24 and 14 mu M respectively. LB-1b also demonstrated hypoglycaemic activity in drug-induced diabetic mice and anti-HIV-1 RT (reverse transcriptase) with an IC50 value of 12.5 mu M. |
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