| 论文题目: | Gracilibacillus kekensis sp nov., a moderate halophile isolated from Keke Salt Lake |
|---|---|
| 作者: | Gao, M; Liu, ZZ; Zhou, YG; Liu, HC; Ma, YC; Wang, L; Chen, SF; Ji, XC |
| 联系作者: | Chen, SF |
| 刊物名称: | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY |
| 期: | |
| 卷: | 62 |
| 页: | 1032-1036 |
| 年份: | 2012 |
| 影响因子: | 2.268 |
| 论文下载: | |
| 摘要: | A novel moderately halophilic bacterium, designated strain K170(T), was isolated from Keke Salt Lake in Qinghai, China. The strain grew with 0-22% (w/v) NaCl, at 4-50 degrees C and at pH 6-11, with optimum growth in 3% (w/v) NaCl, at 40 degrees C and at pH 8. The predominant respiratory quinone was menaquinone 7 (MK-7). The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, aminolipids and glycolipids. The major cellular fatty acids were anteiso-C-15:0, iso-C-15:0 and anteiso-C-17:0. The DNA G+C content was 35.8 mol%. Phylogenetic analysis based on the full-length 16S rRNA gene sequence revealed that strain K170(T) was a member of the genus Gracilibacillus. High levels of 16S rRNA gene sequence similarity were found between strain K170(T) and Gracilibacillus boracfitolerans DSM 17256(T)(973%) and Gracilibacillus thailandensis JCM 15569(T) (97.1%). 16S rRNA gene sequence similarities between strain K170(T) and the type strains of other recognized members of the genus Gracilibacillus were below 97%. The DNA-DNA hybridization values of strain K170(T) with G. boracfitolerans DSM 17256(T) and G. thailandensis JCM 15569(T) were 21.9 % and 34.3%, respectively. On the basis of these results, strain K170(T) is considered to represent a novel species of the genus Gracilibacillus, for which the name Gracilibacillus kekensis sp. nov. is proposed; the type strain is K170(T) (=CGMCC 1.10681(T)= DSM 23178(T)). |
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