| 论文题目: | Role of Itk signalling in the interaction between influenza A virus and T-cells |
|---|---|
| 作者: | Fan, KW; Jia, YP; Wang, S; Li, H; Wu, DF; Wang, GS; Chen, JL |
| 联系作者: | Chen, JL |
| 刊物名称: | JOURNAL OF GENERAL VIROLOGY |
| 期: | |
| 卷: | 93 |
| 页: | 987-997 |
| 年份: | 2012 |
| 影响因子: | 3.363 |
| 论文下载: | |
| 摘要: | Although the T-cell-mediated immune response to influenza virus has been studied extensively, little information is available on the direct interaction between influenza virus and T-cells that pertains to severe diseases in humans and animals. To address these issues, we utilized the BALB/c mouse model combined with primary T-cells infected with A/WSN/33 influenza virus to investigate whether influenza virus has an affinity for T-cells in vivo. We observed that small proportions of CD4(+) T-cells and CD8(+) T-cells in spleen and thymus expressed viral proteins in infected mice. A significant proportion of mouse primary T-cells displayed expression of alpha-2,6 sialic acid-linked influenza virus receptor and were infected directly by influenza A virus. These experiments reveal that there exists a population of T-cells that is susceptible to influenza A virus infection. Furthermore, we employed human Jurkat T-cells to investigate the virus-T-cell interaction, with particular emphasis on understanding whether Itk (interleukin-2-inducible T-cell kinase), a Tec family tyrosine kinase that regulates T-cell activation, is involved in virus infection of T-cells. Interestingly, influenza virus infection resulted in an increased recruitment of Itk to the plasma membrane and an increased level of phospholipase C-gamma 1 (PLC-gamma 1) phosphorylation, suggesting that Itk/PLC-gamma 1 signalling is activated by the virus infection. We demonstrated that depletion of Itk inhibited the replication of influenza A virus, whereas overexpression of Itk increased virus replication. These results indicate that Itk is required for efficient replication of influenza virus in infected T-cells. |
京公网安备 11010502044263号