| 论文题目: | Analysis of the role of transcription factor VAD-5 in conidiation of Neurospora crassa |
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| 作者: | Sun, XY; Yu, LN; Lan, N; Wei, SP; Yu, YF; Zhang, HX; Zhang, XY; Li, SJ |
| 联系作者: | Li, SJ |
| 刊物名称: | FUNGAL GENETICS AND BIOLOGY |
| 期: | 5 |
| 卷: | 49 |
| 页: | 379-387 |
| 年份: | 2012 |
| 影响因子: | 3.737 |
| 论文下载: | |
| 摘要: | Conidiation is the major mode of reproduction in many filamentous fungi. The Neurospora crassa gene vad-5, which encodes a GAL4-like Zn2Cys6 transcription factor, was suggested to contribute to conidiation in a previous study using a knockout mutant. In this study, we confirmed the positive contribution of vad-5 to conidiation by gene complementation. To understand the role of VAD-5 in conidiation, transcriptomic profiles generated by digital gene expression profiling from the vad-5 deletion mutant and the wild-type strain were compared. Among 7559 detected genes, 176 genes were found to be transcriptionally down-regulated and 277 genes transcriptionally upregulated in the vad-5 deletion mutant, using >= 1-fold change as a cutoff threshold. Among the down-regulated genes, four which were already known to be involved in conidiation - fluffy, ada-6, rca-1, and eas - were examined further in a time course experiment. Transcription of each of the four genes in the vad-5 deletion mutant was lower than in the wild-type strain during conidial development. Phenotypic observation of deletion mutants for 132 genes down-regulated by vad-5 deletion revealed that deletion mutants for 17 genes, including fluffy, ada-6, and eas, produced fewer conidia than the wild type. By phenotypic observation of deletion mutants for 211 genes upregulated in the vad-5 deletion mutant, two types of deletion mutants were found. One type, which produced more conidia than the wild-type strain, includes deletion mutants for previously characterized genes cat-2, cat-3, and sah-1 and for a non-characterized gene NCU07221. Deletion mutants of NCU06302 and NCU11090, representing the second type, produced conidia earlier than the wild-type strain. Based on these conidiation phenotypes, we designated NCU07221 as high conidial production-1 (hcp-1) and named NCU06302 and NCU11090 as early conidial development-1 (ecd-1) and ecd-2, respectively. Given the collective results from this study, we propose that VAD-5 exerts an effect on conidiation by activating genes that positively contribute to conidiation as well as by repressing genes that negatively influence conidial development. (C) 2012 Elsevier Inc. All rights reserved. |
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