| 论文题目: | Microbacterium marinum sp nov., isolated from deep-sea water |
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| 作者: | Zhang, LM; Xi, LJ; Ruan, JS; Huang, Y |
| 联系作者: | Huang, Y |
| 刊物名称: | SYSTEMATIC AND APPLIED MICROBIOLOGY |
| 期: | 2 |
| 卷: | 35 |
| 页: | 81-85 |
| 年份: | 2012 |
| 影响因子: | 3.366 |
| 论文下载: | |
| 摘要: | Two Gram-positive, rod-shaped bacterial strains, H101(T) and H207, were isolated from deep sea water collected from South-West Indian Ocean. Phylogenetic analysis of 16S rRNA gene sequences showed that the two strains were closely related to one another (100% similarity), and had the closest relationship with Microbacterium hominis NBRC 15708(T) and Microbacterium insulae KCTC 19247(T) (98.2-98.3% similarities). DNA-DNA hybridization value between strains H101(T) and H207 was 87.2 +/- 3.7%, and the values between the two strains and the closely related type strains were well below 70%. The two strains also shared a number of physiological and biochemical characteristics that were distinct from the closely related species, and grew at 2-37 degrees C, pH 5-11 and 0-8% (w/v) NaCl. Both strains contained MK-12, MK-13 and MK-11 as the detected menaquinones. The peptidoglycan was of type B1 gamma with an interpeptide bridge D-Glu(Hyg) -> Gly(2) -> L-Lys. The major cellular fatty acids were anteiso-C-15:0, anteiso-C-17:0, and iso-C-16:0. Based on the genetic and phenotypic properties, it is proposed that strains H101(T) and H207 be classified as representatives of a novel species of the genus Microbacterium, with the name Microbacterium marinum sp. nov. The type strain is H101(T) (=CGMCC 4.6941(T) = DSM 24947(T)). (C) 2011 Elsevier GmbH. All rights reserved. |
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