| 论文题目: | Over-expression of pcvA involved in vesicle-vacuolar fusion affects the conidiation and penicillin production in Penicillium chrysogenum |
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| 作者: | Xu, XX; Yang, J; An, Y; Pan, YY; Liu, G |
| 联系作者: | Liu, G |
| 刊物名称: | BIOTECHNOLOGY LETTERS |
| 期: | 3 |
| 卷: | 34 |
| 页: | 519-526 |
| 年份: | 2012 |
| 影响因子: | 1.683 |
| 论文下载: | |
| 摘要: | Rab GTPase is required for vesicle-vacuolar fusion during the vacuolar biogenesis in fungi. Rab GTPase-encoding gene, pcvA, was cloned from Penicillium chrysogenum: it contained five introns and its predicted protein contained the conserved Rab GTPase domain involved in GTP-binding and hydrolysis. Over-expression of pcvA significantly stimulated the vesicle-vacuolar fusion but repressed the conidiation and decreased conidial tolerance against thermal stress. Penicillin production was decreased in the pcvA over-expressed strain suggesting that pcvA is involved in vesicle-vacuolar fusion participates in the penicillin biosynthesis in P. chrysogenum. |
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