| 论文题目: | Development of a colorimetric assay for rapid quantitative measurement of clavulanic acid in microbial samples |
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| 作者: | Dai, XD; Xiang, SH; Li, J; Gao, Q; Yang, KQ |
| 联系作者: | Gao, Q |
| 刊物名称: | SCIENCE CHINA-LIFE SCIENCES |
| 期: | 2 |
| 卷: | 55 |
| 页: | 158-163 |
| 年份: | 2012 |
| 影响因子: | 2.024 |
| 论文下载: | |
| 摘要: | We developed a colorimetric assay to quantify clavulanic acid (CA) in culture broth of Streptomyces clavuligerus, to facilitate screening of a large number of S. clavuligerus mutants. The assay is based on a beta-lactamase-catalyzed reaction, in which the yellow substrate nitrocefin (lambda (max)=390 nm) is converted to a red product (lambda (max)=486 nm). Since CA can irreversibly inhibit beta-lactamase activity, the level of CA in a sample can be measured as a function of the A (390)/A (486) ratio in the assay mixture. The sensitivity and detection window of the assay were determined to be 50 mu g L-1 and 50 mu g L-1 to 10 mg L-1, respectively. The reliability of the assay was confirmed by comparing assay results with those obtained by HPLC. The assay was used to screen a pool of 65 S. clavuligerus mutants and was reliable for identifying CA over-producing mutants. Therefore, the assay saves time and labor in large-scale mutant screening and evaluation tasks. The detection window and the reliability of this assay are markedly better than those of previously reported CA assays. This assay method is suitable for high throughput screening of microbial samples and allows direct visual observation of CA levels on agar plates. |
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