| 论文题目: | Bacillus endoradicis sp nov., an endophytic bacterium isolated from soybean root |
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| 作者: | Zhang, YZ; Chen, WF; Li, M; Sui, XH; Liu, HC; Zhang, XX; Chen, WX |
| 联系作者: | Chen, WF |
| 刊物名称: | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY |
| 期: | |
| 卷: | 62 |
| 页: | 359-363 |
| 年份: | 2012 |
| 影响因子: | 2.268 |
| 论文下载: | |
| 摘要: | A Gram-positive, aerobic, motile rod, designated strain CCBAU 05776(T), was isolated from the inner tissues of a healthy soybean (Glycine max L.) root collected from an agricultural field in the countryside of Shijiazhuang city, Hebei Province, China. Phylogenetic analysis of the 16S rRNA gene indicated that this strain was most closely related to Bacillus muralis LMG 20238(T) and Bacillus simplex NBRC 15720(T) with similarity of 96.5 % and 96.3%, respectively, lower than the suggested threshold (97.0%) for separating bacterial species. In phenotypic characterization, the novel strain differed from the two most related species in that it did not hydrolyse casein or starch but could grow on MacConkey agar. It grew between 15 and 45 degrees C and tolerated up to 7% NaCl (w/v). Strain CCBAU 05776(T) grew in media with pH 5.5 to 10 (optimal growth at pH 7.0-8.0). The predominant cellular fatty acids were iso-C-15:0 (40.81%) and C-16:1 omega 7c alcohol (10.61%). The predominant isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C was 40.8 mol% (T-m). DNA DNA relatedness of the novel isolate with B. muralis and B. simplex was 42.4% and 32.7%, respectively. Based upon the consensus of phylogenetic and phenotypic analyses, strain CCBAU 057761 represents a novel species within the genus Bacillus, for which the name Bacillus endoradicis sp. nov. is proposed. The type strain is CCBAU 05776(T) (=LMG 25492(T) =HAMBI 3097(T)). |
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