Nocardioides alpinus sp nov., a psychrophilic actinomycete isolated from alpine glacier cryoconite----中国科学院微生物研究所

科研成果

论文题目:	Nocardioides alpinus sp nov., a psychrophilic actinomycete isolated from alpine glacier cryoconite
作者:	Zhang, DC; Schumann, P; Redzic, M; Zhou, YG; Liu, HC; Schinner, F; Margesin, R
联系作者:	Margesin, R
刊物名称:	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
期:
卷:	62
页:	445-450
年份:	2012
影响因子:	2.268
论文下载:
摘要:	A Gram-positive, non-motile, rod-shaped, psychrophilic actinomycete, designated strain Cr7-14(T), was isolated from alpine glacier cryoconite. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Cr7-14(T) was related to members of the genus Nocardioides and shared highest 16S rRNA gene sequence similarities with the type strains of Nocardioides furvisabuli (98.6 %), Nocardioides ganghwensis (98.2%), Nocardioides oleivorans (98.1 %) and Nocardioides exalbidus (97.6%). The predominant cellular fatty acids of strain Cr7-14(T) were C-17:1 omega 8c (39.5%) and iso-C-16:0 (32.4%). The major menaquinone was MK-8(H-4). The diagnostic diamino acid in the cell-wall peptidoglycan was LL-2,6-diaminopimelic acid. The predominant cell-wall sugars were galactose and rhamnose. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, four unknown glycolipids and two unknown polar lipids. The genomic DNA G + C content was 71.9 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA DNA relatedness data, a novel species, Nocardioides alpinus sp. nov., is proposed, with Cr7-14(T) (=DSM 23325(T)=LMG 26053(T)=CGMCC 1.10697(T)) as the type strain.