| 论文题目: | Characterization of a recombinant (-)gamma-lactamase from Microbacterium hydrocarbonoxydans |
|---|---|
| 作者: | Yang, M; Gao, Q; Wu, S; Wang, JJ; Zheng, GJ |
| 联系作者: | Zheng, GJ |
| 刊物名称: | BIOTECHNOLOGY LETTERS |
| 期: | 2 |
| 卷: | 34 |
| 页: | 275-279 |
| 年份: | 2012 |
| 影响因子: | 1.683 |
| 论文下载: | |
| 摘要: | A (-)gamma-lactamase, Mhg, from Microbacterium hydrocarbonoxydans was over-expressed in E. coli and was characterized after purification. The maximum activity was at pH 8.0 and 60A degrees C and the half life of Mhg was similar to 30 min at 75A degrees C. The enzyme was activated by DTT. The catalytic triad of the (-)gamma-lactamase is comprised of residues Ser98, Asp230, and His259 and an oxyanion hole was formed by Tyr32 and Met99 according to the alignment results. Under native conditions, the (-)gamma-lactamase consists of two 31 kDa homodimers. |
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