Expression, refolding and preliminary X-ray crystallographic analysis of equine MHC class I molecule complexed with an EIAV-Env CTL epitope----中国科学院微生物研究所

科研成果

论文题目:	Expression, refolding and preliminary X-ray crystallographic analysis of equine MHC class I molecule complexed with an EIAV-Env CTL epitope
作者:	Yao, SG; Qi, JX; Liu, J; Chen, R; Pan, XC; Li, XY; Gao, F; Xia, C
联系作者:	Xia, C
刊物名称:	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
期:
卷:	68
页:	20-23
年份:	2012
影响因子:	0.506
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摘要:	In order to clarify the structure and the peptide-presentation characteristics of the equine major histocompatibility complex (MHC) class I molecule, a complex of equine MHC class I molecule (ELA-A1 haplotype, 7-6 allele) with mouse beta 2-microglobulin and the cytotoxic T lymphocyte (CTL) epitope Env-RW12 (RVEDVTNTAEYW) derived from equine infectious anaemia virus (EIAV) envelope protein (residues 195206) was refolded and crystallized. The crystal, which belonged to space group P21, diffracted to 2.3 angstrom resolution and had unit-cell parameters a = 82.5, b = 71.4, c = 99.8 angstrom, beta = 102.9 degrees. The crystal structure contained two molecules in the asymmetric unit. These results should help to determine the first equine MHC class I molecule structure presenting an EIAV CTL epitope.