Protein expression, crystallization and preliminary X-ray crystallographic analysis of chicken interferon-? receptor a chain----中国科学院微生物研究所

科研成果

论文题目:	Protein expression, crystallization and preliminary X-ray crystallographic analysis of chicken interferon-? receptor a chain
作者:	Ping, ZG; Shi, Y; Sun, YL; Ma, LP; Wang, M
联系作者:	Wang, M
刊物名称:	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
期:
卷:	68
页:	41-44
年份:	2012
影响因子:	0.506
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摘要:	The activity of interferon-gamma (IFN-gamma) relies on signal transduction, which is triggered by combination with the receptors interferon-gamma receptor alpha chain (IFNGR1) and beta chain (IFNGR2). Native recombinant chicken IFNGR1 (chIFNGR1; residues 25237) was overexpressed in Escherichia coli, purified by refolding and crystallized using the vapour-diffusion technique. The crystals belonged to space group P6522, with unit-cell parameters a = b = 64.1, c = 216.3 angstrom, alpha = beta = 90, gamma = 120 degrees. The Matthews coefficient and solvent content were calculated as 2.67 angstrom(3) Da(-1) and 53.97%, respectively. X-ray diffraction data for chIFNGR1 were collected to 2.0 angstrom resolution at a synchrotron source.