| 论文题目: | Novel lipolytic genes from microbial metagenomic library of South China Sea marine sediment |
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| 作者: | Yongfei Hu#, Chengzhang Fu#, Yunpeng Huang, Yeshi Yin, Gong Cheng, Fang Lei, Na Lu, Jing Li, Elizabeth Jane Ashforth, Lixin Zhang* and Baoli Zhu* |
| 联系作者: | 朱宝利、张立新 |
| 刊物名称: | FEMS Microbiology Ecology |
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| 年份: | 2010 |
| 影响因子: | 3.598 |
| 论文下载: | |
| 摘要: | Metagenomic cloning is a powerful tool for the discovery of novel genes and biocatalysts from environmental microorganisms. Based on activity screening of a marine sediment microbial metagenomic library, a total of 19 fosmid clones showing lipolytic activity were identified. After subcloning, 15 different lipolytic genes were obtained; their encoded proteins showed 32–68% amino acid identity with proteins in the database. Multiple sequence alignment and phylogenetic tree analysis demonstrated that most of these predicted proteins are new members of known families of bacterial lipolytic enzymes. However, two proteins, FLS18C and FLS18D, could not be assigned to any known family, thus probably representing a novel family of the bacterial lipolytic enzyme. The activity assay results indicated that most of these lipolytic enzymes showed optimum temperature for hydrolysis at 40–50 °C with p-nitrophenol butyrate as a substrate. The lipolytic gene fls18D was overexpressed, and the resulting protein FLS18D was characterized as an alkaline esterase. Furthermore, the whole sequence of fosmid pFL18 containing FLS18C and FLS18D was shotgun sequenced, and a total of 26 ORFs on it were analyzed and annotated. |
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