| 论文题目: | Cellulosilyticum ruminicola gen. nov., sp. nov., isolated from yak rumen contents, and reclassification of Clostridium lentocellum as Cellulosilyticum lentocellum comb. |
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| 作者: | Shichun Cai and Xiuzhu Dong* |
| 联系作者: | 东秀珠 |
| 刊物名称: | International Journal of Systematic and Evolutionary Microbiology |
| 期: | |
| 卷: | 60 |
| 页: | 845-849 |
| 年份: | 2010 |
| 影响因子: | 2.113 |
| 论文下载: | |
| 摘要: | An obligate anaerobic, Gram-staining negative, mesophilic cellulolytic bacterium strain H1T was isolated from the rumen content of yak. Cells were straight to slightly curved rods, 0.8-1.0x3.0-4.0 µm in size, non-motile and capsulated with mucous materials. Elliptical and terminal spores that swelled the cells were produced occasionally. The strain grew at 25-45°C;, optimum at 38°C, and pH range for growth was 6.0-7.8, optimum at pH 6.7. Cellulose, cellobiose, xylan, xylose, and maltose were used as carbon and energy sources, but not glucose. Products from cellulose and cellobiose fermentation were formic acid, acetic acid, CO2 and trace amount of ethanol, lactic and succinic acids. The genomic DNA G+C content was 33.7±1.2 mol%. The predominant fatty acids were C16:0 (27.1 %) and C14:0 (9.2 %). Based on the 16S rRNA gene sequence analysis, strain H1 was affiliated to the clostridial rRNA cluster XIVb and showed the highest 16S rRNA gene sequence similarity to the misclassified species Clostridium lentocellum (96.0%). Strain H1 and C. lentocellum formed a distinct linage of the family Lachnospiraceae. Based on the polyphasic data of strain H1 and Clostridium lentocellum, a novel genus, Cellulosilyticum gen. nov. is proposed, and Cellulosilyticum ruminicola sp. nov. is proposed for strain H1, Clostridium lentocellum is moved to the new genus as Cellulosilyticum lentocellum comb. nov. (type strain ATCC 49066T =DSM 5427T). The type strain of Cellulosilyticum ruminicola sp. nov. is strain H1T (=CGMCC 1.5065T =JCM 14822T). |
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