| 论文题目: | Methanoculleus hydrogenotrophus sp. nov., a novel methanogenic archaeon isolated from Tibetan Plateau in China . |
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| 作者: | Jianqing Tian, Yanfen Wang, Xiuzhu Dong* |
| 联系作者: | 东秀珠 |
| 刊物名称: | International Journal of Systematic and Evolutionary Microbiology |
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| 年份: | 2010 |
| 影响因子: | 2.222 |
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| 摘要: | An obligate anaerobic methanogenic archaeal strain HCT was isolated from Zoige wetland at Tibetan Plateau in China. The strain was isolated through constructing an artificial butyrate-degrading consortium of co-culturing with a syntrophic bacterium, Syntrophomonas erecta subsp. sporosyntropha. Strain HCT was irregular coccoid with the diameter of 0.8-2 µm and singly arranged and only utilized H2/CO2 for growth and methane production. Growth occurred at temperature 18-45 °C, and optimally around 37 °C. The pH for growth was 5.0-8.5, and optimal growth was around pH 6.6. The G+C content of the genomic DNA was 60.2 mol%. 16S rRNA gene sequence homology analysis indicated that strain HCT was affiliated to the Genus of Methanoculleus at sequence similarities of 94.8-97.2% with the existing members. However, strain HCT distinguished from the described Methanoculleus species by not using formate for growth and methane formation, nor requiring acetate as growth factor. Based on the phylogenetic analysis and phenotypic characteristics, a novel species Methanoculleus hydrogenitrophicus sp. nov. is proposed, with strain HCT as the type strain (=CGMCC1.5146T=JCM 16311T). |
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