Identification of HLA-A24-Binding Peptides of Mycobacterium tuberculosis Derived Proteins with Beta 2m Linked HLA-A24 Single Chain Expressing Cells.----中国科学院微生物研究所

科研成果

论文题目:	Identification of HLA-A24-Binding Peptides of Mycobacterium tuberculosis Derived Proteins with Beta 2m Linked HLA-A24 Single Chain Expressing Cells.
作者:	Jie Ding Yan Wang,Tingting Cheng, Xiaowei Chen, and Bin Gao*
联系作者:	高斌
刊物名称:	Immunological Investigations
期:	2
卷:	39
页:	103-113
年份:	2010
影响因子:	1.754
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摘要:	Tuberculosis is caused by an intracellular pathogen Mycobacterium tuberculosis (Mtb) and poses a persistent threat to global health. MHC class I-restricted CD8 cytotoxic T lymphocytes (CTL) are essential for protective immunity to Tuberculosis. Information for CTL epitopes derived from Mtb is desirable for vaccine design and assessment of T cell responses. However, the knowledge about CTL epitopes of Mtb, particularly those non-A2 HLA alleles restricted is rare. In this study, beta-2-microglobulin (beta 2m, beta(2)m) linked HLA-A24 single chain was expressed on RMA-S cell line defective in the endogenous antigen processing and applied for screening of peptides which could stabilize the HLA-A24 complex on the cell surface. From a group of peptides predicted as binders by a computer algorithm, five peptides were shown to bind to HLA-A24 protein on the cell surface. As comparison we have also identified a dozen Mtb proteins derived peptides that bind to HLA-A2 specifically. The cell line and HLA binders present here would be useful for further identification of CD8 restricted Mtb epitopes.