Dongia mobilis gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from a sequencing batch reactor for treatment of malachite green effluent.----中国科学院微生物研究所

科研成果

论文题目:	Dongia mobilis gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from a sequencing batch reactor for treatment of malachite green effluent.
作者:	Ying Liu, Jinghua Jin, Yinghao Liu, Yuguang Zhou and Zhipei Liu*
联系作者:	刘志培
刊物名称:	International Journal of Systematic and Evolutionary Microbiology
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卷:
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年份:	2010
影响因子:	2.222
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摘要:	A Gram-negative, strictly aerobic and heterotrophic, non-spore-forming bacterial strain, designated LM22T, was isolated from activated sludge in a sequencing batch reactor for treatment of malachite green effluent. Cells of strain LM22T were slightly curved to straight rods with 0.3-0.5 µm wide and 0.6-1.0 µm long, motile by a single polar flagellum. Negative for oxidase and catalase activities, and phototrophic growth. Internal membrane system and bacteriochlorophyll were absent. Growth occured at temperatures 20-40 oC (optimum at 30-35 oC) and pH 6.0-10.0 (optimum at 7.0-7.5). NaCl was not obligatory for growth and strain LM22T tolerated up to 2.0 % NaCl (w/v, optimum in 0.5 %). The major ubiquinone was Q-10. The major fatty acids (>10 % of the total) were C18:1 7c (32.9 %), C19:0 cyclo 8c (18.7 %), C16:0 (12.1 %) and C16:0 2OH (10.5 %). Phylogenetic analyses of 16S rRNA gene sequences showed that Inquilinus limosus AU476T was the closest relative (similarity of 90.4%). The DNA G+C content was 65.6 mol %. On basis of phenotypic, chemotaxonomic and phylogenetic data, strain LM22T was considered to represent a novel genus and species of the family Rhodospirillaceae, for which the name Dongia mobilis gen. nov., sp. nov. is proposed. The type strain is LM22T (=CGMCC 1.7660T = JCM 15798T).