Mop-like replication and the formation of replication foci of archaeal virus SIRV2 carrying linear dsDNA with hairpin ends

报告题目：Mop-like replication and the formation of replication foci of archaeal virus SIRV2 carrying linear dsDNA with hairpin ends

报告人：Xu Peng 教授

单位：哥本哈根大学 生物系 丹麦古菌中心

时间：2016年4月8日 上午9：00

地点：微生物所A202会议室

主持人： 黄力 研究员

摘要： Viruses have been found to employ diverse replication strategies. In contrast to the two well-defined re2plication modes employed by some viruses, the θ replication and the σ replication, much less is known about the more complex replication involving multimeric and highly branched viral replicative intermediates. Here, we describe a novel replication strategy exploited by the crenarchaeal rudivirus SIRV2 carrying linear dsDNA with hairpin ends. We find that SIRV2 forms large, multimeric and branched replication intermediates with unusual “mop-like” structures, resulted from strand-displacement replication with multiple consecutive reinitiation events from one parental template. Moreover, our data suggest the involvement of a diversity of replication modes in SIRV2 replication including strand-displacement from both termini of the linear dsDNA genome, rolling-circle mode of replication and bidirectional replication from a circular dimeric intermediate. Further, SIRV2 forms replication centers in the infected host cells where host replication proteins such as DNA polymerase DpoI and PCNA concentrate, mimicking eukaryotic viral factories.

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