Integration of Aromatic Acid Chemotaxis and Catabolism

　　报告题目: Integration of Aromatic Acid Chemotaxis and Catabolism

　　报 告 人: Prof. Rebecca Ehrlich Parales

　　报告人单位: Department of Microbiology and Molecular Genetics, University of California, Davis

　　报告时间: 2015年11月20日(星期五)3:00 ~ 4:00 pm

　　报告地点: A203会议室

　　主 持 人: 刘双江 研究员

　　报告摘要:

　　Aromatic and hydroaromatic compounds that are metabolized through the β-ketoadipate catabolic pathway serve as chemoattractants forPseudomonas putida F1. We recently identified PcaY, the methyl-accepting chemotaxis protein responsible for chemotaxis to aromatic acids by screening a series of P. putida F1 mutants each lacking one of the genes encoding the 18 putative methyl-accepting chemotaxis proteins (MCPs). PcaY is required for metabolism–independent chemotaxis to vanillate, vanillin, 4-hydroxybenzoate, benzoate, protocatechuate, quinate, shikimate, as well as ten substituted benzoates that do not serve as growth substrates for P. putida F1. The chemotactic response is induced during growth on aromatic compounds, and an analysis of a pcaY-lacZ fusion revealed that pcaY is expressed in the presence of β-ketoadipate, a common intermediate in the pathway. pcaY expression also required the transcriptional activator PcaR, indicating that pcaY is a member of the pca regulon, which includes three unlinked gene clusters that encode five enzymes required for the conversion of 4-hydroxybenzoate to TCA cycle intermediates, as well as the major facilitator superfamily transport protein PcaK. A chimeric PcaY biosensor was generated and used as a proof-of-concept for the generation of chimeric MCPs that will allow the identification of ligands for of chemoreceptors of unknown function.

　　报告人简介：

　　Professional Experience

　　2003-2006 Assistant Professor, Section of Microbiology, University of California Davis

　　2006-2010 Associate Professor, Department of Microbiology, University of California Davis

　　2010-2013 Professor, Department of Microbiology, UC Davis

　　2013- Professor, Department of Microbiology and Molecular Genetics, UC Davis

　　Selected Professional Activities

　　2011-2016 Editor, Applied and Environmental Microbiology

　　2012-2017 Editor, Microbiology

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