Anaerobic transformation of halogenated aromatic

　　报告人单位：Department of Isotope Biogeochemistry,

　　Helmholtz Centre for Environmental Research, Germany

　　报告时间：2014年7月21日(星期一)15:30-16:30

　　报告地点： 微生物所A102会议室

　　主持人：杜文斌 研究员

　　报告摘要：Bacteria have been identified using halogenated

　　aromatic compounds as an electron acceptor in anaerobic respiration. The

　　process is significant for the halogen element cycle and can be exploited for

　　the remediation of contaminated soil and ground water. We are studying Dehalococcoides species that can be found world-wide. The cells

　　can transform chlorinated benzenes, dioxins, PCBs, chlorophenols and many

　　compounds more. Genome sequencing has shown that the cells were exposed to an

　　evolutionary pressure for a long time leading to the development of many

　　reductive dehalogenase operons in the genome. Biochemical characterization of

　　the reductive dehalogenase enzymes has demonstrated the enzyme function for

　　some of the dehalogenase genes and supplied evidence for a new protein-bound

　　electron transport providing the energy for the organism. Proteomic and

　　isotopic analyses have illuminated specific functions in the anabolism of Dehalococcoides species. The research has impact for

　　biotechnological implementation in remediation but also for understanding

　　microbial life in deep-marine sediments where Dehalococcoides-related Chloroflexi are highly abundant. A

　　recent project revealed large parts of a single-cell genome isolated from

　　marine sediments and indicates many physiological functions previously unknown.