微生物代谢工程与生物催化剂开发研究组

 组长：陶勇　博士、研究员

 研究组研究方向

     以代谢工程为基础的新型生物催化剂开发与利用 

 研究组研究内容及意义

我们的研究包括以下几个方面：

(1) 工业酶制剂与蛋白的重组表达策略：

从多学科入手，对大肠杆菌、毕赤酵母等重组蛋白高效表达的细胞工厂进行系统的研究、改造及开发利用。基于对重组表达宿主菌转录、翻译及蛋白质折叠等生理过程的深入研究，并充分利用已有的基因组信息，对细胞工厂进行系统有针对性的改造。开发出具有不同遗传背景的重组表达宿主菌，在不同的重组蛋白与工业酶的表达过程中应用。

(2) 生物催化剂的开发利用：

利用代谢工程和系统生物学等手段，开发出具有不同应用价值的重组微生物菌株（包括大肠杆菌、酵母等）进行生物催化。如利用生物质生产具有高附加值的化合物和保健品、替代化学途径、环境治理等。

 研究组长

研究组长：陶勇 博士 研究员 

电话：010-64807419

传真：010-64807419

电子邮件：taoyong@im.ac.cn

通讯地址：北京市朝阳区北辰西路1号院3号，中国科学院微生物研究所

邮政编码：100101

 主要学习及工作经历

学习经历 

1981年-1985年，南京大学，获得生物化学学士学位。

1985年-1988年，中国科学院微生物研究所，获得生物化学及酶学硕士学位。

1991年-1995年，美国的Rutgers大学，获得生物化学及分子生物学博士学位。

工作经历

1988年至1991年：中国科学院微生物研究所，助理研究员。

1995年至1997年：美国洛克菲勒大学，博士后。

1997年至2008年：美国杜邦农业化学公司，生物化学研究员、资深生物化学研究员。

2008年至2010年：美国杜邦公司研发中心，资深研究员。

2010年至今：中国科学院微生物研究所，研究员，博士生导师。工业微生物与生物技术研究室主任。

 研究团队

工作人员     

刘伟丰 助理研究员

林白雪 博士后

 代表性论文

 （1）2020年 

1.   Zhang Y, Zhou H,Tao Y, Lin B (2020) Reconstitution of the ornithine cycle with arginine:glycine amidinotransferase to engineer Escherichia coli into an efficient whole-cell catalyst of guanidinoacetate. ACS synthetic biology2020,doi:10.1021/acssynbio.0c00138 

2.   Feng J, Li R, Zhang S, Bu Y, Chen Y, Cui Y, Lin B, Chen Y,Tao Y, Wu B, Bioretrosynthesis of FunctionalizedN-Heterocycles from Glucose via One-Pot Tandem Collaborations of Designed Microbes. Advanced Science 2020,2001188. 

3. Gao Q, Chen H, Wang W, Huang J,Tao Y, Lin B (2020) Menaquinone-7 production in engineered Escherichia coli. World journal of microbiology & biotechnology2020,36(9):132-132. 

4.   Wang L, Piao X, Cui S, Hui M,Tao Y(2020) Enhanced production of beta-alanine through co-expressing two different subtypes ofl-aspartate-alpha-decarboxylase. Journal of Industrial Microbiology & Biotechnology2020,47(6-7):465-474. 

5.   Zhu, J.; Yang, W.; Wang, B.; Liu, Q.; Zhong, X.; Gao, Q.; Liu, J.; Huang, J.; Lin, B.;Tao, Y., Metabolic engineering ofEscherichia colifor efficient production of L-alanyl-L-glutamine. Microb Cell Fact2020,19 (1): 129. 

6.   You, R.; Wang, L.; Shi, C.; Chen, H.; Zhang, S.; Hu, M.;Tao, Y., Efficient production of myo-inositol inEscherichia colithrough metabolic engineering. Microb Cell Fact2020,19 (1): 109. 

7.   Yao, J.; Li, J.; Xiong, D.; Qiu, Y.; Shi, G.; Jin, J. M.;Tao, Y.; Tang, S. Y., Development of a highly efficient and specific L-theanine synthase. Appl Microbiol Biotechnol2020,104 (8): 3417-3431. 

8.   Yao, J.; He, Y.; Su, N.; Bharath, S. R.;Tao, Y.; Jin, J. M.; Chen, W.; Song, H.; Tang, S. Y., Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps. Nat Commun2020,11 (1): 1515. 

9.  Xu, X.; Zhong, H.; Liu, W.;Tao, Y., Extension of Genetic Marker List Using Unnatural Amino Acid System: An Efficient Genomic Modification Strategy inEscherichia coli. Front Bioeng Biotechnol2020,8: 145. 

10. Wang, L.; Song, L.; He, X.; Teng, F.; Hu, M.;Tao, Y., Production of isofloridoside from galactose and glycerol using α-galactosidase fromAlicyclobacillus hesperidum. Enzyme Microb Technol2020,134: 109480. 

11. Wang, L.; Piao, X.; Cui, S.; Hu, M.;Tao, Y., Enhanced production of β-alanine through co-expressing two different subtypes of L-aspartate-α-decarboxylase. J Ind Microbiol Biotechnol2020. 

12.Ma, C.; Mu, Q.; Wang, L.; Shi, Y.; Zhu, L.; Zhang, S.; Xue, Y.;Tao, Y.; Ma, Y.; Yu, B., Bio-production of high-purity propionate by engineering L-threonine degradation pathway inPseudomonas putida. Appl Microbiol Biotechnol2020,104 (12): 5303-5313. 

 （2）2019年 

1.   Zhang, S.; Yang, W.; Chen, H.; Liu, B.; Lin, B.;Tao, Y., Metabolic engineering for efficient supply of acetyl-CoA from different carbon sources inEscherichia coli. Microb Cell Fact2019,18 (1): 130. 

2.   Wang, T.; Teng, K.; Liu, Y.; Shi, W.; Zhang, J.; Dong, E.; Zhang, X.;Tao, Y.; Zhong, J.,Lactobacillus plantarumPFM 105 Promotes Intestinal Development Through Modulation of Gut Microbiota in Weaning Piglets. Front Microbiol2019,10: 90. 

3.   Wang, J.; Zhao, H.; Zhao, G.; Chen, D.;Tao, Y.; Wu, S., Enhancing the atypical esterase promiscuity of the γ-lactamase Sspg fromSulfolobus solfataricusby substrate screening. Appl Microbiol Biotechnol2019,103 (10): 4077-4087. 

4.   Teng, F.; You, R.; Hu, M.; Liu, W.; Wang, L.;Tao, Y., Production of d-glucuronic acid from myo-inositol usingEscherichia coliwhole-cell biocatalyst overexpressing a novel myo-inositol oxygenase fromThermothelomyces thermophile. Enzyme Microb Technol2019,127: 70-74. 

5.   Piao, X.; Wang, L.; Lin, B.; Chen, H.; Liu, W.;Tao, Y., Metabolic engineering ofEscherichia colifor production of L-aspartate and its derivative β-alanine with high stoichiometric yield. Metab Eng2019,54: 244-254. 

6.   Mu, Q.; Cui, Y.; Tian, Y.; Hu, M.;Tao, Y.; Wu, B., Thermostability improvement of the glucose oxidase fromAspergillus nigerfor efficient gluconic acid production via computational design. Int J Biol Macromol2019,136: 1060-1068. 

7.  Liu, B.; Xiang, S.; Zhao, G.; Wang, B.; Ma, Y.; Liu, W.;Tao, Y., Efficient production of 3-hydroxypropionate from fatty acids feedstock inEscherichia coli. Metab Eng2019,51: 121-130. 

8.  Hou, Y.; Chen, S.; Wang, J.; Liu, G.; Wu, S.;Tao, Y., Isolating promoters fromCorynebacterium ammoniagenesATCC 6871 and application in CoA synthesis. BMC Biotechnol2019,19 (1): 76. 

9.   Chen, W.; Yao, J.; Meng, J.; Han, W.;Tao, Y.; Chen, Y.; Guo, Y.; Shi, G.; He, Y.; Jin, J. M.; Tang, S. Y., Promiscuous enzymatic activity-aided multiple-pathway network design for metabolic flux rearrangement in hydroxytyrosol biosynthesis. Nat Commun2019,10 (1): 960. 

10. 刘波;陶勇,生物制造“细胞工厂”的设计与组装.生物工程学报2019,35 (10): 1942-1954. 

11.张译文;林白雪;陶勇,氨甲酰磷酸不同合成途径在大肠杆菌中的比较.微生物学通报2019,46 (09): 2111-2120. 

 （3）2018年 

1.   Yang, Y.; Zhang, Y.; Zhang, S.; Chen, Q.; Ma, K.; Bao, L.;Tao, Y.; Yin, W.; Wang, G.; Liu, H., Identification and Characterization of a Membrane-Bound Sesterterpene Cyclase fromStreptomyces somaliensis. J Nat Prod2018,81 (4): 1089-1092. 

2.   Wang, T.; Teng, K.; Liu, G.; Liu, Y.; Zhang, J.; Zhang, X.; Zhang, M.;Tao, Y.; Zhong, J.,Lactobacillus reuteriHCM2 protects mice against EnterotoxigenicEscherichia colithrough modulation of gut microbiota. Sci Rep2018,8 (1): 17485. 

3.   Ni, K.; Zhao, J.; Zhu, B.; Su, R.; Pan, Y.; Ma, J.; Zhou, G.;Tao, Y.; Liu, X.; Zhong, J., Assessing the fermentation quality and microbial community of the mixed silage of forage soybean with crop corn or sorghum. Bioresour Technol2018,265: 563-567. 

4.   Lu, Y.; Wang, L.; Teng, F.; Zhang, J.; Hu, M.;Tao, Y., Production of myo-inositol from glucose by a novel trienzymatic cascade of polyphosphate glucokinase, inositol 1-phosphate synthase and inositol monophosphatase. Enzyme Microb Technol2018,112: 1-5. 

5.   Liu, Z.; Jin, Y.; Liu, W.;Tao, Y.; Wang, G., Crystal structure of IspF fromBacillus subtilisand absence of protein complex assembly amongst IspD/IspE/IspF enzymes in the MEP pathway. Biosci Rep2018,38 (1): BSR20171370. 

6.   Li, R.; Wijma, H. J.; Song, L.; Cui, Y.; Otzen, M.; Tian, Y.; Du, J.; Li, T.; Niu, D.; Chen, Y.; Feng, J.; Han, J.; Chen, H.;Tao, Y.; Janssen, D. B.; Wu, B., Computational redesign of enzymes for regio- and enantioselective hydroamination. Nat Chem Biol2018,14 (7): 664-670. 

7.   Han, J.; Gao, Q. X.; Zhang, Y. G.; Li, L.; Mohamad, O. A. A.; Rao, M. P. N.; Xiao, M.; Hozzein, W. N.; Alkhalifah, D. H. M.;Tao, Y.; Li, W. J., Transcriptomic and Ectoine Analysis of HalotolerantNocardiopsis gilvaYIM 90087^TUnder Salt Stress. Front Microbiol2018,9: 618. 

8.   Bu, Y.; Cui, Y.; Peng, Y.; Hu, M.; Tian, Y.;Tao, Y.; Wu, B., Engineering improved thermostability of the GH11 xylanase fromNeocallimastix patriciarumvia computational library design. Appl Microbiol Biotechnol2018,102 (8): 3675-3685. 

9.   钟瑾;倪奎奎;杨军香;玉柱;陶勇,我国饲用草产品加工技术的现状及展望.科学通报2018,63 (17): 1677-1685. 

10.  谭华荣;陶勇;钟瑾,我国放线菌和乳链菌肽产生菌遗传学研究的先行者——薛禹谷.微生物学报2018,58 (08): 1505-1506. 

11.  刘姣;刘伟丰;陶勇;马延和,一种大肠杆菌快速连续CRISPR基因组编辑的新策略.微生物学通报2018,45 (08): 1621-1631. 

 （4）2017年 

1.  Yang, Y. L.; Zhang, S.; Ma, K.; Xu, Y.; Tao, Q.; Chen, Y.; Chen, J.; Guo, S.; Ren, J.; Wang, W.;Tao, Y.; Yin, W. B.; Liu, H., Discovery and Characterization of a New Family of Diterpene Cyclases in Bacteria and Fungi. Angew Chem Int Ed Engl2017,56 (17): 4749-4752. 

2.  Xie, Z.; Zhang, Z.; Cao, Z.; Chen, M.; Li, P.; Liu, W.; Qin, H.; Zhao, X.;Tao, Y.; Chen, Y., An external substrate-free blue/white screening system inEscherichia coli. Appl Microbiol Biotechnol2017,101 (9): 3811-3820. 

3.  Wang, P.; Yang, X.; Lin, B.; Huang, J.;Tao, Y., Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol. Metab Eng2017,44: 143-149. 

4.  Ni, K.; Wang, F.; Zhu, B.; Yang, J.; Zhou, G.; Pan, Y.;Tao, Y.; Zhong, J., Effects of lactic acid bacteria and molasses additives on the microbial community and fermentation quality of soybean silage. Bioresour Technol2017,238: 706-715. 

5.   Lv, X.; Cao, H.; Lin, B.; Wang, W.; Zhang, W.; Duan, Q.;Tao, Y.; Liu, X. W.; Li, X., Synthesis of Sialic Acids, Their Derivatives, and Analogs by Using a Whole-Cell Catalyst. Chemistry2017,23 (60): 15143-15149. 

6.  Lin, B.;Tao, Y., Whole-cell biocatalysts by design. Microb Cell Fact2017,16 (1): 106. 

7.  Li, H.; Liang, C.; Chen, W.; Jin, J. M.; Tang, S. Y.;Tao, Y., Monitoring in vivo metabolic flux with a designed whole-cell metabolite biosensor of shikimic acid. Biosens Bioelectron2017,98: 457-465. 

8.  Fan, K.; Lin, B.;Tao, Y.; Yang, K., Engineering deacetoxycephalosporin C synthase as a catalyst for the bioconversion of penicillins. J Ind Microbiol Biotechnol2017,44 (4-5): 705-710. 

9.  Cui, Q.; Zhou, F.; Liu, W.;Tao, Y., Avermectin biosynthesis: stable functional expression of branched chain α-keto acid dehydrogenase complex fromStreptomyces avermitilisinEscherichia coliby selectively regulating individual subunit gene expression. Biotechnol Lett2017,39 (10): 1567-1574. 

10. 金应福;韩莉;张莎莎;李世忠;刘伟丰;陶勇,通过番茄红素环化酶的优化构建β-胡萝卜素高产菌株.生物工程学报2017,33 (11): 1814-1826. 

11.  唐双焱;陶勇,新时期下的酶工程研究.广西科学2017,24 (01): 3. 

 （5）2016年 

1.  Zhang, Y.; Li, Y.; Zhang, Y.; Wang, Z.; Zhao, M.; Su, N.; Zhang, T.; Chen, L.; Wei, W.; Luo, J.; Zhou, Y.; Xu, Y.; Xu, P.; Li, W.;Tao, Y., Quantitative Proteomics Reveals Membrane Protein-Mediated Hypersaline Sensitivity and Adaptation in HalophilicNocardiopsis xinjiangensis. J Proteome Res2016,15 (1): 68-85. 

2.  Lin, B.;Tao, Y., Production of Sialic Acid and Its Derivatives by Metabolic Engineering ofEscherichia coli.In《Quality Living Through Chemurgy and Green Chemistry》(ISBN: 978-3-662-53702-2), Springer:2016;pp 301-318. (Book Chapter) 

3.  Lin, B.; Qiao, Y.; Shi, B.;Tao, Y., Polysialic acid biosynthesis and production inEscherichia coli: current state and perspectives. Applied microbiology and biotechnology2016,100 (1): 1-8. 

4. Ke, C. R.; Yang, X. W.; Rao, H. X.; Zeng, W. C.; Hu, M. R.;Tao, Y.; Huang, J. Z., Whole-cell conversion of l-glutamic acid into gamma-aminobutyric acid by metabolically engineeredEscherichia coli. Springerplus2016,5: 591. 

5.  Jin, Y.; Liu, Z.; Li, Y.; Liu, W.;Tao, Y.; Wang, G., A structural and functional study on the 2-C-methyl-d-erythritol-4-phosphate cytidyltransferase (IspD) fromBacillus subtilis. Sci Rep2016,6: 36379. 

6. Chu, X.; Han, J.; Guo, D.; Fu, Z.; Liu, W.;Tao, Y., Characterization of UDP-glucose dehydrogenase fromPasteurella multocidaCVCC 408 and its application in hyaluronic acid biosynthesis. Enzyme Microb Technol2016,85: 64-70. 

7.  牟庆璇;胡美荣;陈飞;江贤章;陶勇;黄建忠,采用混合策略提高葡萄糖氧化酶在毕赤酵母中的表达水平.生物工程学报2016,32 (07): 986-990. 

8.  韩莉;张莎莎;王博军;刘京国;刘伟丰;王艳萍;陶勇,重组产番茄红素大肠杆菌工程菌株中异戊烯异构酶(IDI)基因的筛选.食品工业科技2016,37 (13): 137-142. 

9.  高庆华;胡美荣;吴芳彤;陶勇;王云鹏;罗同阳;胡常英,点青霉葡萄糖氧化酶基因的克隆及其酶学性质研究.生物技术通报2016,32 (07): 152-159. 

 （6）2015年 

1. Zhang, S.; Zhao, X.;Tao, Y.; Lou, C., A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method. J Biol Eng2015,9: 23. 

2. Lin, B.; Fan, K.; Zhao, J.; Ji, J.; Wu, L.; Yang, K.;Tao, Y., Reconstitution of TCA cycle with DAOCS to engineerEscherichia coliinto an efficient whole cell catalyst of penicillin G. Proc Natl Acad Sci U S A2015,112 (32): 9855-9. 

3.  Li, C.; Ying, L. Q.; Zhang, S. S.; Chen, N.; Liu, W. F.;Tao, Y., Modification of targets related to the Entner-Doudoroff/pentose phosphate pathway route for methyl-D-erythritol 4-phosphate-dependent carotenoid biosynthesis inEscherichia coli. Microb Cell Fact2015,14: 117. 

4.  He, Y. Z.; Gong, J.; Yu, H. Y.;Tao, Y.; Zhang, S.; Dong, Z. Y., High production of ectoine from aspartate and glycerol by use of whole-cell biocatalysis in recombinantEscherichia coli. Microb Cell Fact2015,14: 55. 

5.  Chen, F.;Tao, Y.; Jin, C.; Xu, Y.; Lin, B. X., Enhanced production of polysialic acid by metabolic engineering ofEscherichia coli. Appl Microbiol Biotechnol2015,99 (6): 2603-11. 

6.  Chen, F.; Chen, X. Z.; Su, X. Y.; Qin, L. N.; Huang, Z. B.;Tao, Y.; Dong, Z. Y., An Ime2-like mitogen-activated protein kinase is involved in cellulase expression in the filamentous fungusTrichoderma reesei. Biotechnol Lett2015,37 (10): 2055-62. 

7. Chen, F.; Chen, X. Z.; Qin, L. N.;Tao, Y.; Dong, Z. Y., Characterization and homologous overexpression of an N-acetylglucosaminidase Nag1 fromTrichoderma reesei. Biochem Biophys Res Commun2015,459 (2): 184-188. 

8.  冯凡;许杨;陶勇;刘伟丰;林白雪,提高大肠杆菌通过MVA途径合成异戊二烯.生物工程学报2015,31 (07): 1073-1081. 

9.  陈飞;胡美荣;江贤章;陶勇;黄建忠,共表达分子伴侣PDI和Ero1对灰盖鬼伞过氧化物酶在毕赤酵母中表达的影响.生物工程学报2015,31 (12): 1682-1689. 

10.  张丛丛;陈彩霞;陈笑;温雅;晏礼明;陶勇,全细胞催化法生产N-乙酰神经氨酸的研究进展.生物技术通报2015,31 (04): 175-183. 

11.  徐婷;崔占鸿;钟瑾;骆爱群;陶勇;刘书杰,青海省部分地区人工种植燕麦青贮乳酸菌的筛选.江苏农业科学2015,43 (02): 205-208. 

12.  王瑶;许杨;陈楠;徐欣怡;刘伟丰;陶勇,在大肠杆菌中利用SCLM系统进行高效率λ-Red基因敲除/整合的新策略.微生物学通报2015,42 (04): 699-711. 

 （7）2014年 

1.  Pan, X.; Chen, X.; Su, X.; Feng, Y.;Tao, Y.; Dong, Z., Involvement of SpoVG in hemolysis caused byBacillus subtilis. Biochem Biophys Res Commun2014,443 (3): 899-904. 

2.   Hu, M.; Zhou, X.; Shi, Y.; Lin, J.; Irfan, M.;Tao, Y., Essential role of the N- and C-terminals of laccase fromPleurotus floridaon the laccase activity and stability. Appl Biochem Biotechnol2014,174 (5): 2007-17. 

3.   张瑶;徐平;李文均;陶勇,放线菌蛋白质组学研究进展.生物工程学报2014,30 (07): 1044-1058. 

4.   石艺平;周雪;胡美荣;林剑辉;陶勇;黄建忠,不同信号肽对毕赤酵母表达漆酶的影响.微生物学报2014,54 (12): 1446-1452. 

5.   黄建忠;林剑辉;石艺平;胡美荣;陶勇,大豆过氧化物酶在毕赤酵母中功能表达.微生物学通报2014,41 (09): 1850-1855. 

6.   郭东会;韩剑;刘伟丰;傅震洲;朱启忠;陶勇,两种UDP-葡萄糖脱氢酶对透明质酸生物转化的影响.生物工程学报2014,30 (11): 1691-1700. 

7.   付金衡;赵健;林白雪;许杨;陶勇,全细胞催化生产苯乙酰-7-氨基-3-脱乙酰氧基头孢烷酸的条件优化.生物工程学报2014,30 (11): 1781-1785. 

8.   安莉颖;易欣欣;秦丽娜;施思;陶勇;伍红;董志扬,里氏木霉RutC30常温常压等离子体(ARTP)诱变筛选pyr4基因缺陷型菌株及转化系统的建立.菌物学报2014,33 (06): 1272-1280. 

 （8）2013年 

1.   Yu, F.; Liu, X.;Tao, Y.; Zhu, K., High saturated fatty acids proportion inEscherichia colienhances the activity of ice-nucleation protein fromPantoea ananatis. FEMS Microbiol Lett2013,345 (2): 141-6. 

2.  Tao, Y.; Gutteridge, S.; Benner, E. A.; Wu, L.; Rhoades, D. F.; Sacher, M. D.; Rivera, M. A.; Desaeger, J.; Cordova, D., Identification of a critical region in theDrosophila ryanodinereceptor that confers sensitivity to diamide insecticides. Insect Biochem Mol Biol2013,43 (9): 820-8. 

3.   Lin, B. X.; Zhang, Z. J.; Liu, W. F.; Dong, Z. Y.;Tao, Y., Enhanced production of N-acetyl-D-neuraminic acid by multi-approach whole-cell biocatalyst. Appl Microbiol Biotechnol2013,97 (11): 4775-84. 

4.  张新杰;贺鹏;陶勇;杨毅,基于筛选标记整合拷贝增加的酿酒酵母外源蛋白高效表达.微生物学报2013,53 (11): 1195-1204. 

5.  许杨;陈芳;林白雪;陶勇,全细胞催化生产N-乙酰神经氨酸的条件优化.微生物学通报2013,40 (08): 1331-1338. 

6.  曲璟秋;刘翠花;刘伟丰;陶勇;朱坤,应用基因敲除快速构建大肠杆菌突变体改造脂肪酸代谢途径.微生物学报2013,53 (06): 608-614. 

7.   刘伟丰;陶勇,蛋白质预算:合成生物学的成本标尺.生物工程学报2013,29 (08): 1123-1132. 

  （9）2012年 

1.  Tao, Y.; Cheng, Q.; Kopatsis, A. D., Metabolic engineering for acetate control in large scale fermentation.In《Microbial Metabolic Engineering》(ISBN: 1064-3745), 2011/12/07 ed.;2012;Vol. 834, pp 283-303. (Book Chapter: 18) 

2.  Qin, L. N.; Cai, F. R.; Dong, X. R.; Huang, Z. B.;Tao, Y.; Huang, J. Z.; Dong, Z. Y., Improved production of heterologous lipase inTrichoderma reeseiby RNAi mediated gene silencing of an endogenic highly expressed gene. Bioresour Technol2012,109: 116-22. 

3.  董欣睿;秦丽娜;陶勇;黄建忠;董志扬,糙皮侧耳Pleurotus ostreatus漆酶POXA1在里氏木霉中的高效表达及酶学性质.微生物学报2012,52 (07): 850-856. 

4.   胡开蕾;韩剑;刘伟丰;王艳萍;陶勇,嗜热脱氮土壤芽孢杆菌β-葡萄糖苷酶的克隆与重组表达及其酶学性质研究.微生物学通报2012,39 (07): 891-900. 

 （10）2010年以前 

1.  Chou-Fei, W.; Yang, X.; Yong, T.; Ji-Yan, Y., Establishment of hypoglycemic agent screening method based on human glucokinase. Biomedical and Environmental Sciences2009,22 (1): 62-69. 

2.   Wu, J.;Tao, Y.; Zhang, M.; Howard, M. H.; Gutteridge, S.; Ding, J., Crystal structures ofSaccharomyces cerevisiaeN-myristoyltransferase with bound myristoyl-CoA and inhibitors reveal the functional roles of the N-terminal region. Journal of Biological Chemistry2007,282 (30): 22185-22194. 

3.   Cordova, D.; Benner, E.; Sacher, M.; Rauh, J.; Sopa, J.; Lahm, G.; Selby, T.; Stevenson, T.; Flexner, L.; Caspar, T.; Ragghianti, J.; Gutteridge, S.; Rhoades, D. F.; Wu, L.; Smith, R. M.;Tao, Y., Elucidation of the mode of action of Rynaxypyr^TM, a selective ryanodine receptor activator; Ohkawa H, Miyagawa H, Lee PW; Pesticide Chemistry: Crop Protection.In《Pesticide Chemistry: Crop Protection, Public Health, Environmental Safety》(ISBN: 9783527316632), Ohkawa, H.; Miyagawa, H.; Lee, P., Eds.2007;pp 121-126. (Book Chapter: 12) 

4.   Cordova, D.; Benner, E. A.; Sacher, M. D.; Rauh, J. J.; Sopa, J. S.; Lahm, G. P.; Selby, T. P.; Stevenson, T. M.; Flexner, L.; Gutteridge, S.; Rhoades, D. F.; Wu, L.; Smith, R. M.;Tao, Y., Anthranilic diamides: A new class of insecticides with a novel mode of action, ryanodine receptor activation. Pesticide Biochemistry and Physiology2006,84 (3): 196-214. 

5.   Gutteridge, S.; Pember, S. O.; Wu, L.;Tao, Y.; Walker, M., Fungal site of action determination: Integration with high-volume screening and lead progression.In《New Discoveries in Agrochemicals》(ISBN: 1947-5918), ACS Publications:2005;Vol. 892, pp 132-141. (Book Chapter: 12) 

6.   Howard, M. H.; Cenizal, T.; Gutteridge, S.; Hanna, W. S.;Tao, Y.; Totrov, M.; Wittenbach, V. A.; Zheng, Y. J., A novel class of inhibitors of peptide deformylase discovered through high-throughput screening and virtual ligand screening. J Med Chem2004,47 (27): 6669-72. 

7.  Zheng, Y.-J.;Tao, Y.; Zhang, W.; Jordan, D. B., Inhibition of fungal aldose reductase. Protein and Peptide Letters2001,8 (5): 407-412. 

8.   Guermah, M.;Tao, Y.; Roeder, R. G., Positive and negative TAF_IIfunctions that suggest a dynamic TFIID structure and elicit synergy with traps in activator-induced transcription. Mol Cell Biol2001,21 (20): 6882-94. 

9.  Xiao, H.;Tao, Y.; Roeder, R. G., The human homologue ofDrosophilaTRF-proximal protein is associated with an RNA polymerase II-SRB complex. J Biol Chem1999,274 (7): 3937-40. 

10. Kimura, H.;Tao, Y.; Roeder, R. G.; Cook, P. R., Quantitation of RNA polymerase II and its transcription factors in an HeLa cell: little soluble holoenzyme but significant amounts of polymerases attached to the nuclear substructure. Mol Cell Biol1999,19 (8): 5383-92. 

11. Xiao, H.;Tao, Y.; Greenblatt, J.; Roeder, R. G., A cofactor, TIP30, specifically enhances HIV-1 Tat-activated transcription. Proc Natl Acad Sci U S A1998,95 (5): 2146-51. 

12.Purrello, M.; Di Pietro, C.; Viola, A.; Rapisarda, A.; Stevens, S.; Guermah, M.;Tao, Y.; Bonaiuto, C.; Arcidiacono, A.; Messina, A.; Sichel, G.; Grzeschik, K. H.; Roeder, R., Genomics and transcription analysis of human TFIID. Oncogene1998,16 (12): 1633-8. 

13.Oelgeschl？ger, T.;Tao, Y.; Kang, Y. K.; Roeder, R. G., Transcription activation via enhanced preinitiation complex assembly in a human cell-free system lacking TAF_IIs. Mol Cell1998,1 (6): 925-31. 

14.Martinez, E.; Ge, H.;Tao, Y.; Yuan, C. X.; Palhan, V.; Roeder, R. G., Novel cofactors and TFIIA mediate functional core promoter selectivity by the human TAF_II150-containing TFIID complex. Mol Cell Biol1998,18 (11): 6571-83. 

15. Tao, Y.; Guermah, M.; Martinez, E.; Oelgeschl？ger, T.; Hasegawa, S.; Takada, R.; Yamamoto, T.; Horikoshi, M.; Roeder, R. G., Specific interactions and potential functions of human TAF_II100. J Biol Chem1997,272 (10): 6714-21. 

16.Yan, Y. P.;Tao, Y.; Chen, K. Y., Molecular cloning and functional expression of human deoxyhypusine synthase cDNA based on expressed sequence tag information. Biochem J1996,315 (Pt 2): 429-34. 

17. 还连栋;陶勇;田宇清;何松;薛禹谷,利用PCR技术克隆乳链菌肽前体基因.生物工程学报1995, (04): 389-391. 

18. 还连栋;陶勇;何松;田宇清;薛禹谷,乳链菌肽高产菌株的选育及其基因定位.微生物学报1995, (05): 364-367. 

19.Tao, Y.; Chen, K. Y., Molecular cloning and functional expression ofNeurosporadeoxyhypusine synthase cDNA and identification of yeast deoxyhypusine synthase cDNA. J Biol Chem1995,270 (41): 23984-7. 

20. Tao, Y.; Chen, K. Y., Purification of deoxyhypusine synthase fromNeurospora crassato homogeneity by substrate elution affinity chromatography. J Biol Chem1995,270 (1): 383-6. 

21.Tao, Y.; Skrenta, H. M.; Chen, K. Y., Deoxyhypusine synthase assay based on the use of polyhistidine-tagged substrate and metal chelate-affinity chromatography. Anal Biochem1994,221 (1): 103-8. 

22.Tao, Y.; Chen, K. Y., PCR-based cloning of the full-length Neurospora eukaryotic initiation factor 5A cDNA: polyhistidine-tagging and overexpression for protein affinity binding. Biochem J1994,302 ( Pt 2) (Pt 2): 517-25. 

23. 陶勇;严自正;张树政,溜曲霉β-N-乙酰氨基己糖苷酶的催化特性.微生物学报1992,32 (06): 405-411. 

24.李梅;陶勇;严自正;张树政,溜曲霉β-N-乙酰氨基己糖苷酶的化学修饰.微生物学报1992,32 (02): 115-118. 

25.陶勇, Nisin及其在食品防腐方面的应用.食品科学1991, (02): 53-57. 

26.严自正;陶勇;程秀兰;孙晋武;张树政, β-N-乙酰氨基己糖苷酶产生菌的筛选与产酶条件.微生物学报1990,30 (02): 122-128. 

27. 陶勇;严自正;孙晋武;程秀兰;张树政,溜曲霉β-N-乙酰氨基己糖苷酶的纯化与性质.微生物学报1990,30 (04): 259-266. 

28.陶勇, DNA分子模型与复制方式并不矛盾.生命的化学1987, (1): 21. 

29. 曹幼琴;林玉琴;郑金荣;陶勇;李梅,扬州平山五泉矿泉水的微生物学评价.南京大学学报(自然科学版)1987, (03): 422-429. 

 授权专利

 （1）2020年 

1.  于瑶;韩剑;林白雪;陶勇.一种检测待测样本中目标蛋白的含量的方法.授权专利号：ZL 201710306828.3,授权日期：2020-01-21. 

2.   刘伟丰;刘波;崔倩倩;赵广;陶勇.产3-羟基丙酸的重组菌及其制备方法与应用.授权专利号：ZL 201710247568.7,授权日期：2020-01-31. 

3.   胡美荣;王雷;滕斐;陶勇.一种生产葡萄糖醛酸的方法及其专用工程菌.授权专利号：ZL 201710790108.9,授权日期：2020-05-12. 

（2）2019年 

1.   林白雪;袁慎键;杨巍;陶勇.海藻糖的生产方法.授权专利号：ZL 201610249035.8,授权日期：2019-07-26. 

2.   黄建忠;柯崇榕;杨欣伟;钟璐芳;任洋;陶勇.生产吡咯喹啉醌的细菌及其应用.授权专利号：ZL 201610512635.9,授权日期：2019-11-01. 

3.   胡美荣;邱媛媛;彭颖;陶勇.重组载体、含有其的工程菌及在产α-酮戊二酸的应用.授权专利号：ZL 201510923517.2,授权日期：2019-12-10. 

4.   胡美荣;马宏;贾振华;黄媛媛;宋水山;陶勇;张立文.一种奎宁酮还原酶RrQR的制备方法及其在制备(R)-3-奎宁醇中应用.授权专利号：ZL 201510237818.X,授权日期：2019-09-27. 

5.   葛书华;晏礼明;陶勇;康美华;傅深展;杨勇;张子娟.一种转化脂肪酸衍生物生产长链二元酸的方法.授权专利号：ZL 201510176594.6,授权日期：2019-06-14. 

6.   陶勇;韩剑;郭东会.一种高效产透明质酸的重组菌的构建方法.授权专利号：ZL201410415767.0,授权日期：2019-07-09. 

7.   胡美荣;彭颖;陶勇.产α-熊果苷的基因工程菌及其构建方法和应用.授权专利号：ZL201510171160.7,授权日期：2019-05-28. 

（3）2018年 

1.   郑翔;胡美荣;刘春卯;陶勇;薛刚;马清河;秦艳梅;胡常英;杨何宝;李江燕;董聪.枯草工程菌Bacillus subtilis及其构建以及利用其生产脱毛酶制剂的方法.授权专利号：ZL 201510890412.1,授权日期：2018-08-31. 

2.  陈彩霞;晏礼明;陶勇;葛书华.一种从ɑ-熊果苷发酵液中分离ɑ-熊果苷的方法.授权专利号：ZL 201510345857.1,授权日期：2018-12-25. 

3.  赖小勤;晏礼明;杨勇;张子娟;陈远童;陶勇;傅深展.长链二元酸生产菌株及其制备方法和应用.授权专利号：ZL 201410176116.0,授权日期：2018-01-12. 

4.   赖小勤;晏礼明;杨勇;张子娟;陈远童;傅深展;陶勇.长链二元酸生产菌株及其制备方法和应用.授权专利号：ZL 201410182202.2,授权日期：2018-01-12. 

5.   赖小勤;晏礼明;杨勇;陈远童;傅深展;陶勇.长链二元酸生产菌株及其制备方法和应用.授权专利号：ZL 201410175544.1,授权日期：2018-01-12. 

6.  陈楠;刘伟丰;林白雪;陶勇.高生物量和/或高生长速度重组菌及其构建方法与应用.授权专利号：ZL 201410167194.4,授权日期：2018-09-20. 

（4）2017年 

1.   柯崇榕;黄建忠;陶勇;杨欣伟;胡美荣;饶焕新;曾文超.产γ-氨基丁酸的重组菌及其构建方法与应用.授权专利号：ZL 201510229161.2,授权日期：2017-09-22. 

2.   黄建忠;江贤章;周芬;刘洪娇;祁峰;张明亮;陶勇.温度敏感型载体pBBR1MCS-2-Ts3及其应用.授权专利号：ZL 201510340762.0,授权日期：2017-11-03. 

3.  黄建忠;江贤章;周芬;刘洪娇;祁峰;张明亮;陶勇.温度敏感型载体pBBR1MCS-2-Ts1及其应用.授权专利号：ZL 201510340926.X,授权日期：2017-11-03. 

4.   林白雪;赵健;杨克迁;陶勇.产头孢菌素G的重组菌及其构建方法与应用.授权专利号：ZL 201410032447.7,授权日期：2017-09-22. 

5.  赖小勤;晏礼明;杨勇;葛书华;陈远童;陶勇;傅深展.长链二元酸生产菌株及其制备方法和应用.授权专利号：ZL 201410175556.4,授权日期：2017-02-08. 

（5）2016年以前 

1.   贺鹏;张新杰;陶勇.一种酿酒酵母整合型表达载体.授权专利号：ZL 201310003066.1,授权日期：2016-05-04. 

2.  胡美荣;周雪;陶勇.一种漆酶突变体及其编码基因与应用.授权专利号：ZL 201310700591.9,授权日期：2015-08-12. 

3.  林白雪;张子娟;陶勇.产N-乙酰神经氨酸的基因工程菌及其构建方法与应用.授权专利号：ZL ZL201210349623.0,授权日期：2014-07-16. 

4.  Gutteridge, S.; Caspar, T.; Cordova, D.; Rauh, J. J.;Tao, Y.; Wu, L. Isolation and use of ryanodine receptors.授权专利号：US 8551694B2,授权日期：2013-10-08. 

5.  Cahoon, R. E.;Tao, Y.Plant 1-deoxy-D-xylulose 5-phosphate reductoisomerase.授权专利号：US 8461322B2,授权日期：2013-06-11. 

6.  Orozco Jr, E. M.; Cahoon, R. E.; Bruce, W. B.;Tao, Y.; Weng, Z. Auxin transport proteins.授权专利号：US 7943753B2,授权日期：2011-05-17. 

7.  Caspar, T.; Cordova, D.; Gutteridge, S.; Rauh, J. J.; Wu, L.;Tao, Y.Ryanodine receptor polypeptides.授权专利号：US 8101715B2,授权日期：2012-01-24. 

8.   Casper, T.; Cordova, D.; Gutteridge, S.; Rauh, J. J.; Smith, R. M.; Wu, L.;Tao, Y.Isolation and use of ryanodine receptors.授权专利号：US 7655395B2,授权日期：2010-02-02. 

9.  Cahoon, R. E.; Lee, J. M.;Tao, Y.Plant 1-deoxy-D-xylulose 5-phosphate reductoisomerase.授权专利号：US 7842855B2,授权日期：2010-11-30. 

10.Cahoon, R. E.;Tao, Y.Plant 1-deoxy-D-xylulose 5-phosphate reductoisomerase.授权专利号：US 7282359B2,授权日期：2007-10-16. 

11. Cahoon, R. E.;Tao, Y.Rice 1-deoxy-D-xylulose 5-phosphate synthase and DNA encoding thereof.授权专利号：US 7195887B2,授权日期：2007-03-27. 

12. Orozco Jr, E. M.; Weng, Z.; Bruce, W. B.; Cahoon, R. E.;Tao, Y.Auxin transport proteins.授权专利号：US 7422901B2,授权日期：2008-09-09. 

13.  Tao, Y.Soybean 1-deoxy-D-xylulose 5-phosphate synthase and DNA encoding thereof.授权专利号：US 7132250B2,授权日期：2006-11-07. 

14.  Gutteridge, S.; Caspar, T.; Cordova, D.;Tao, Y.; Wu, L.; Smith, R. M. Nucleic acids encoding ryanodine receptors.授权专利号：US 7205147B2,授权日期：2007-04-17. 

15.  Gutteridge, S.; Caspar, T.; Cordova, D.; Rauh, J. J.; Smith, R. M.; Wu, L.;Tao, Y.Ryanodine receptor polypeptides.授权专利号：US 7498408B2,授权日期：2009-03-03. 

16.  Tao, Y.Soybean 1-deoxy-D-xylulose 5-phosphate synthase and DNA encoding thereof.授权专利号：US 6558915B1,授权日期：2003-05-06. 

17.  Hitz, W. D.; Maxwell, C. A.;Tao, Y.Polynucleotides encoding indole-3-gylcerol phosphate synthase from soybean.授权专利号：US 6482631B1,授权日期：2002-11-19. 

 申请专利